Abstract：Objective To observe the effect of Qiji shenkang granule total flavonoids on the secretion of fibronectin
and collagen type IV and to investigate the role of the target by Qiji shenkang granule of total flavonoids extract
on chronic kinney disease at different time points. Methods Through the pharmacology of serum method ,we
got pharmacological serum of the Qiji shenkang granule total flavonoids high does group and the Qiji shenkang
granule total flavonoids low does groups.Angiotensin II was used as a stimulating factor to culture rat glomerular
mesangial cells, which cause the accumulation of extracellular matrix. The expression of secretion of fibronectin
and collagen IV were tested by enzyme-linked immunosorbent assay (ELISA). Results At different time
points in (24, 48,72) hours, the contents of IV collagen in the high dose group and low were different.
high dose group :(4.635±0.645)μmol/L,(4.645±0.555)μmol/L,(5.170±0.420)μmol/L, low dose group: (5.095±0.355)
μmol/L,(4.945±0.465)μmol/L,(5.340±0.340)μmol/L. Fibronectin high dose group :(246.5±15.0)μmol/L,(245.0±11.0)
μmol/L,(195.6±11.0)μmol/L；low dose group: (256.0±12.0)μmol/L,(256.0±12)μmol/L,(223.6±37.0)μmol/L.
Compared with angiotensin II group,there were significant differences between them (P <0.01), and showing a certain
dose-effect relationship. But the treatment group showed no statistical difference. Conclusion Qiji Shenkang granule
total flavonoids by inhibiting the overexpression of extracellular matrix to delay the development of chronic kidney
disease. The target was clear about Qiji Shenkang granule total flavonoids.